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Biological and Physico-Chemical Surveillance

Biological Assessment of Water Quality

In the presence of pollution, characteristic and well-documented changes are induced in the flora and fauna of rivers and streams. Particularly well documented are the changes brought about by organic pollution in the macroinvertebrate community i.e., the immature aquatic stages of aerial insects (mayflies, stoneflies etc.) together with Crustacea (e.g. shrimps), Mollusca (e.g. snails and bivalves), Oligochaeta (worms) and Hirudinea (leeches). The changes which occur are due to the varying sensitivities of the different components of the community to the stresses caused by pollution. It is known that similar organisms inhabit similar habitats and that the most sensitive species inhabit the riffle areas. It is also well known that community diversity declines in the presence of pollution and that sensitive species are progressively replaced by more tolerant forms as pollution increases. Ideally, all the components of the aquatic biota (the micro-and macro-fauna and flora) should be utilised but in practice macroinvertebrate community analysis is found to be satisfactory for routine water quality monitoring purposes.

For the purposes of the EPA assessment procedure benthic macroinvertebrates have been divided into five arbitrary 'Indicator Groups' as follows: Group A, the sensitive forms, Group B, the less sensitive forms, Group C, the tolerant forms, Group D, the very tolerant forms and Group E, the most tolerant forms. These groups, and their relationships with the Biotic Index (Q values) are set out below.

In contrast to physico-chemical surveys which extend throughout the year, biological surveys are usually undertaken in the summer-autumn period (June-October) when flows are likely to be relatively low and water temperatures highest. Surveys during this period are likely, therefore, to coincide with the worst conditions to be expected in those reaches affected by waste inputs. Biological material for examination is obtained by a 'kick' sampling technique in the faster- flowing areas (riffles) of the river or stream and the examination and assessment of water quality is made on site. Having determined the relative proportions of the various organisms in a sample, water quality can be inferred by a comparison of this data with that which might be expected from unpolluted habitats of the type under investigation. Other relevant factors such as the intensity of algal and/or weed development, water turbidity, bottom siltation, substratum, current speed and water depth, DO saturation and water temperature, are also taken into account in the assessment procedure.

Relationships between water quality and macroinvertebrate community structure are usually described by means of a numerical scale of values. Such a compression of biological information inevitably results in a loss of meaningful information but some such procedure is essential if this information is to be meaningful to non-biologists. The EPA scheme of Biotic Indices or Quality (Q) Values and its relationship to water quality is set out here.

'Q' Value

Community Diversity

Water Quality

Condition *

Q5

High

Good

Satisfactory

Q4

Reduced

Fair

Satisfactory

Q3

Much Reduced

Doubtful

Unsatisfactory

Q2

Low

Poor

Unsatisfactory

Q1

Very Low

Bad

Unsatisfactory

* 'Condition' refers to the likelihood of interference with beneficial or potential beneficial uses.

The intermediate indices Q1-2, 2-3, 3-4 and 4- 5 are also used to denote transitional conditions. The scheme mainly reflects the effects of biodegradable organic wastes (i.e. deoxygenation and eutrophication) but where a toxic effect is apparent or suspected the suffix '0' is added to the biotic index (e.g. Q 1/0, 2/0 or 3/0) and attention is sometimes drawn to siltation or atypical effects by appending an asterix to the biotic index. The scheme may be further simplified as shown by the classification set out below:-

Biotic Index

Quality Status

Quality Class

Q5, 4-5, 4

Unpolluted

Class A

Q3-4

Slightly Polluted

Class B

Q3, 2-3

Moderately Polluted

Class C

Q2, 1-2, 1

Seriously Polluted

Class D

Class A waters are those in which problems relating to existing or potential uses are unlikely to arise; they are, therefore, regarded as being in a 'satisfactory' condition. Classes B, C and D are to a lesser or greater extent 'unsatisfactory' in this regard. For example, the main characteristic of Classes B and C waters is eutrophication which may interfere with the amenity, abstraction or fisheries uses of such waters.

Biological and Physico-Chemical Surveillance - Physico-Chemical Assessment of Water Quality

For the assessment of organic pollution the more commonly measured parameters include DO, BOD, Ammonia, Oxidised Nitrogen (Nitrites plus Nitrates) and Phosphates. Continuous records of concentration and flow would form the ideal basis for water quality assessment but in practice this is impossible for financial, technical and logistical reasons. Reliance must, therefore, be placed on discrete samples because such samples constitute only a minute fraction of the whole body of water under investigation and because they are only representative of conditions at the particular time of sampling the interpretation of data arising from such samples requires great care.

Unlike the biological assessment of water quality, where the incidence and intensity of pollution is based on the degree to which the chosen organism association deviates from its expected natural diversity, the physico-chemical assessment is usually based on a comparison of the measurements made with water quality criteria or with standards derived from such criteria. The setting of national standards for water, sewage and other effluents by the Minister for the Environment is provided for under the Local Government (Water Pollution) Act, 1977 and the Environmental Protection Agency Act, 1992. Water quality guidelines have been issued by the Minister on the advice of a technical committee (Technical Committee on Effluent and Water Quality Standards, 1979). In addition, legally binding standards for water quality in Ireland arise from various EC Directives. Of particular relevance in the present context are the 'Surface Water' and 'Freshwater Fish' Directives (C.E.C., 1975, 1978). The former deals with the quality requirements of waters used as sources of public supply while the latter sets standards for waters harbouring game or coarse fisheries, although these are legally binding only in the case of 'designated' waters. Both of these Directives are now the subject of National Regulations, (Minister for the Environment, 1988, 1989). A digest of these standards and guidelines for the more important of the physico-chemical parameters of pollution by organic wastes as appropriate to fishery salmonid waters is set out below:

Freshwater Fish Regulations

Water Quality Guidelines

Dissolved Oxygen (DO):
50% of samples > 9mg/l O2

50% of samples > 9mg/l O2

95% of samples > 6mg/l O2

No sample < 4 mg/l O2

Biochemical Oxygen Demand (BOD):

< 5 mg/l

< 4mg/l

Ammonia:

< 0.02 mg/l NH3 or

< 0.02 mg/l NH3

< 0.016 mg/l N*

< 0.02 mg/l N*

< 0.8 mg/l N**

*=unionised **=total

These limits are more stringent than those applicable to the same parameters in abstraction waters receiving standard treatment, as set out in the 'Surface Water' Regulations. The same position holds in the case of most other water quality parameters so that the suitability of waters for fisheries is usually a good assurance of their suitability for abstraction and for many other uses. The major exceptions are nitrate and microbiological quality in which cases even high levels of contamination will not directly affect fish life.

Biological and Physico-Chemical Surveillance - Advantages and Shortcomings of the two Approaches

Physico-chemical techniques have the merit of being precise, discriminatory and quantitative and they are, therefore, essential if unpolluted waters are to be chemically typed or if pollutants in water are to be identified and their concentrations quantified. Information of this type is essential to good water management as it provides the basic information required by licensing authorities for the assessment of compliance by licensed discharges with prescribed standards. With regard to general water quality monitoring, however, and particularly where a large number of clean rivers are to be monitored - as in this country - a distinct disadvantage of a purely chemical approach is the cost: whereas just two biological samples per annum (winter and summer) would normally provide a reasonably accurate assessment of average water quality, a considerably greater number of physico - chemical samples would normally be required to achieve such an assessment with the same degree of confidence.

A knowledge of the types of pollutants likely to be present is a prerequisite for effective chemical monitoring. With the increasing complexity of many industrial effluents this may prove difficult if not impossible in certain circumstances. Furthermore, if a discharge is irregular or surreptitious there is a good chance that it will not be detected at all by routine chemical monitoring programmes. Since benthic macroinvertebrate communities respond to a wide range of water quality characteristics and pollutants and because they can reflect the effects of mixed pollutants these shortcomings can often be overcome by biological analysis.

A disadvantage of the biological approach is that, although capable of detecting ecological change, indicative of water quality change, it does not identify the specific cause of a change: for this physico-chemical analysis is essential, especially in the case of toxic pollution. It should also be pointed out that whilst water indicated to be of poor quality on biological grounds is suspect for most uses, water indicated to be of good quality on such grounds, although acceptable for most uses including fisheries, may not always be free from pathogens or harmful trace organics and may not therefore be acceptable as drinking water. Assessment of this aspect requires specific microbiological and physico-chemical tests. Finally, in assessing water quality from data involving benthic communities, due recognition must be given to the influences of other ecological factors such as depth and flow rate, substratum type, the influence of shading and seasonal changes in life cycle.

From the foregoing it may be appreciated that both physico-chemical and biological water quality assessment techniques have their own particular applications, advantages and disadvantages so that only by a combination of both may the limitations of each be overcome and a thorough understanding of the total situation be gained. The advantages and shortcomings of the two approaches are summarised below.

Biological and Physico-Chemical Surveillance - Comparison of Biological and Chemical Water Quality Assessment Techniques

REALM

PERFORMANCE

Chemical

Biological

Precision

Good

Poor

(Pollutant concentration assessment)

Discrimination

Good

Poor

(Pollutant identification)

Measure of Effects

No

Yes

Cost

High

Low

Single Sample Value

Poor

Good


Conclusion : Combination of both techniques preferable to either alone.


General Characteristics of the various Biological Quality Classes.

Quality Classes

Class A

Class B

Class C

Class D

Quality Ratings

Q5

Q4

Q3-4

Q3

Q2

Q1

Pollution Status

Pristine, Unpolluted

Unpolluted

Slight Pollution

Moderate Pollution

Heavy Pollution

Gross Pollution

Organic Waste Load

None

None

Light

Considerable

Heavy

Excessive

Maximum B.O.D.

Low (< 3mg/l)

Low (< 3mg/l)

Occasionally elevated

High at times

Usually High

Usually very high

Dissolved Oxygen

Close to 100% at all times

80%-120%

Fluctuates from <80% to >120%

Very unstable, Potential fish-kills

Low, sometimes zero

Very low, often zero

Annual median PO4

0.015 mg/l

0.03 mg/l

0.045 mg/l

0.07 mg/l

> 0.1 mg/l

> 0.1 mg/l

Siltation

None

May be light

May be light

May be considerable

Usually heavy

Usually very heavy and anaerobic

'Sewage Fungus'

Never

Never

Never

May be some

Usually abundant

May be abundant

Filamentous Algae

Limited Development

Considerable growth, diverse communities

Luxuriant growths, typically Cladophora

Excesssive growths, typically Cladophora

Usually abundant

None

Macrophytes

Diverse communities, limited growths

Diverse Communnities, Considerable Growths

Reduced diversity, luxuriant growths

Limited diversity, excessive growths

Tolerant species only, may be abundant

Most tolerant forms, minimal diversity

Water Quality

Highest quality

Fair Quality

Variable quality

Doubtful quality

Poor quality

Bad quality

Abstraction Potential

Suitable for all

Suitable for all

Potential problems

Advanced treatment

Low grade abstractions

Extremely limited

Fishery Potential

Game fisheries

Good game fisheries

Game fish at risk

Coarse fisheries

Fish usually absent

Fish absent

Amentiy Value

Very high

High

Considerable

Reduced

Low

Zero

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