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Project Code [2009-ET-MS-9-S2]

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Project title

Application of novel enzymes derived from thermoacidophiles to second-generation biofuel production.

Primary Funding Agency

Environmental Protection Agency

Co-Funding Organisation(s)

n/a

Lead Organisation

University of Limerick (UL)

Lead Applicant

Gary Walsh

Project Abstract

Novel cellulase and xylanase enzymes, maximally active at pH ~ 1.0 and 60-90oC, will be identified from thermoacidophilic microorganisms, cloned & produced by recombinant means. The enzymes will be used in application-relevant studies of the production of bioethanol from lignocellulosic feedstocks. Their use in feedstock pre-treatment regimes should allow significant, quantifiable reductions in acid and energy levels currently applied in this step. Consequent environmental (greener production), safety, bioprocess and ultimately economic benefits could significantly advance the prospects of environmentally sustainable bioethanol production from lignocellulosics. Expected outputs include:1. An alternative methodology for the pre-treatment of lingnocellulosic material during bioethanol production2. 4 conference abstracts (2 national, 2 international)3. 2-3 research articles in appropriate environmental/biotech journals4. Potentially 1 patent application5. Summary of work presented at a level comprehensible by members of the general public uploaded on a group research webpage.6. Research capacity building training of 1 post doc in the area of enzyme based environmental biotechnology and provision for follow on research proposals/industrial interaction

Grant Approved

�354,539.30

Research Hub

Green and Circular Economy

Research Theme

Waste and Resources Management

Start Date

01/10/2009

Initial Projected Completion Date

01/10/2012