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Project Code [2007-FS-ET-9-M5]
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Project title
Recombinant DNA approaches to enhance activity of the pathway for degradation of the toxic pollutant styrene in the bioreactor isolate P. putida CA-3: a biotechnologically significant metabolic route.
Primary Funding Agency
Environmental Protection Agency
Co-Funding Organisation(s)
n/a
Lead Organisation
University College Cork (UCC)
Lead Applicant
Mark O'Mahony
Project Abstract
Pseudomonas putida CA-3 is a bioreactor isolate capable of degrading the toxic aromatic pollutant styrene. However, in addition to styrene mineralisation to CO2 and H2O, via acetyl-CoA, this strain is also capable of redirecting styrene catabolism to biodegradable plastic production, (i.e. poly-hydroxyalkanoate), under appropriate growth conditions. This capability has considerable biotechnological significance as it facilitates the conversion of toxic waste to an eco-friendly, value added product. The current project seeks to enhance the ability of P. putida CA-3 to degrade styrene by investigating the flux through the pathway, identifying bottlenecks and applying recombinant DNA approaches to overcome them.
Grant Approved
�166,920.00
Research Hub
Green and Circular Economy
Research Theme
Waste and Resources Management
Initial Projected Completion Date
01/10/2009