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Project Code [2006-PhD-ET-14]
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Project title
Environmental diagnostics: biomolecular interactions at nanostructured surfaces
Primary Funding Agency
Environmental Protection Agency
Co-Funding Organisation(s)
n/a
Lead Organisation
National University of Ireland Galway (NUIG)
Lead Applicant
Donal Leech
Project Abstract
This project aims to develop a novel environmental diagnostic platform for the detection of microbial contamination in environmental samples. The platform is based on the tethering of probe DNA sequences coding for microbial contaminants with redox mediator on nano-structured electrode surfaces. The electrochemical signal is developed by interaction of an enzyme-avidin label with biotinylated DNA at the surface. The enzyme in the presence of the surface-immobilised mediator provides an amplified current signal which allows detection of minute levels of DNA. The performance of the diagnostic assays following optimisation of mediator electrode and immobilisation protocol will be evaluated in real samples.
Grant Approved
�87,800.00
Research Hub
Green and Circular Economy
Research Theme
Waste and Resources Management
Initial Projected Completion Date
01/02/2010